23 Jul Induction-enhanced tissue specific promoter and application thereof
Abstract
The invention relates to an induction-enhanced tissue specific promoter and application thereof, belonging to the technical field of plant genetic engineering. A promoter (BPHP, i.e. Bph14 promoter) for Oryza Sativa L. genes resistant to brown plant hoppers is separated from Oryza Sativa L. and is cloned. The promoter has a nucleotide sequence shown as SEQ ID No. 1, can control Oryza Sativa L. genes resistant to the brown plant hoppers to be specifically expressed in vascular bundle tissues and can be expressed in a way that the feeding induction of the brown plant hoppers is enhanced. The promoter contains BS1EGCCR cis-acting elements and can enable the genes to be specifically expressed in plant vascular bundle tissues. Moreover, the promoter has defense-related response cis-acting elements which can be combined with WRKY transcription factors, and the gene expression is increased when Oryza Sativa L. is fed by the brown plant hoppers or is infected by pathogenic bacteria. The promoter can be used as the promoter for the specific expression in the vascular bundle tissues and can be used as the brown plant hopper and pathogenic bacteria induction-enhanced promoter.
Year: 2010
Country: CN
Doc No: 101792761