23 Jul In-vitro three-dimensional isolated culture and storage method for hCDMSC (human chorion-derived mesenchymal stem cell) for clinical adoptive therapy
Abstract
The invention relates to an in-vitro three-dimensional isolated culture and storage method for an hCDMSC (human chorion-derived mesenchymal stem cell) for clinical adoptive therapy. Tissue construction and in-vitro cell culture belong to the popular science and technology field with great importance in the field of regenerative medicine stem cell treatment. The cell for the clinical adoptive therapy is taken as a prospective medical means. The invention is characterized by adopting the in-vitro three-dimensional isolated culture and storage method for the hCDMSC for the clinical adoptive therapy, the high-purity hCDMSC for the clinical therapy is acquired, a screening and attached culture technology which is never publicly reported and adopts hCDMSC highly-simulated in-vivo ECM (extracellular matrix) is designed and developed under the guidance of ECM theories. The serum-free culture system keeps primitive undifferentiated stem cells, stem cells meeting the standard of ISCT (International Society for Cellular Therapy) are acquired, and the activity and the passage capacity of the stem cells are very high; according to the gradient domestication culture theory adopting multi-combination culture solutions, the hCDMSC is endowed with safety and stability guarantee for the adoptive therapy application, is the scientific and technological base for stem cell treatment developed by a clinical drug design and development medical institution and is also a seeded stem cell with prospect in the field of tissue engineering.
Year: 2016
Country: CN
Doc No: 105713869