23 Jul In vitro construction method for salivary glands organs and acinus
Abstract
The invention discloses an in vitro construction method for biological engineering salivary glands organs and acinus based on human minor salivary gland adult stem cells belonging to the technical field of tissue and organ reconstruction. The in vitro construction method specifically is a method for obtaining organoids with salivary gland structures and functions by mixed cultivation of mesenchymal stem cells and epithelial stem/progenitor cells of the human minor salivary glands, or a method for obtaining acinar-like tissues with salivary gland structures and functions by individual cultivation of epithelial stem/progenitor cells of the human minor salivary glands. The method has the advantages of being wide in sampling source, easy to cut, and small in trauma; a donor site in a mouth is private; materials are drawn from adult tissues; and autoplastic transplantation can be carried out through amplification in vitro. The method has a wide application prospect in growth and development research of in vitro or in vivo salivary glands, research on a salivary gland disease model, research on physiological functions and pathological changes of the saliva and treatment of salivary gland diseases.
Year: 2015
Country: CN
Doc No: 104877964