23 Jul Immortal human bone marrow matrix stem cell line and its establishing process
Abstract
The present invention is immortal human bone marrow matrix stem cell line and its establishing process. The primary human bone marrow matrix stem cell from normally aborted fetus bone marrow tissue is single cell cloned and cultured in a finite dilution process to purify primary cell and transfected with pCIneo-hTERT gene for immortalizing. The human bone marrow matrix stem cell line is extracorporeal cultured, may be infinitely cloned and proliferated and is normal cytologic cell without conversion cell or cancer cell characteristic. The cell may be directionally induced and differentiated into bone cell, cartilage cell or cardiac muscle-like cell and has multiple direction differentiating latent and adult stem cell characteristic. It may be applied in biological engineering field. The present invention provides standard cell line for the biological research of biological behavior of human bone marrow matrix stem cell, platform for basic tissue engineering research, enough seed cells for clinical application and effective method for relevant research.
Year: 2006
Country: CN
Doc No: 1810957