23 Jul Human umbilical cord mesenchymal stem cell culture method and stem cell chondrogenic differentiation culture method
Abstract
The invention relates to a human umbilical cord mesenchymal stem cell culture method and a stem cell chondrogenic differentiation culture method. According to the human umbilical cord mesenchymal stemcell culture method, low-intensity pulsed ultrasonic stimulation is given to the culturing of human umbilical cord mesenchymal stem cells. The human umbilical cord mesenchymal stem cell culture method and the chondrogenic differentiation culture method, provided by the invention, can both increase the cell proliferation rate under an equivalent comparative culture condition. A Human hUC-MSCs in vitro culture and chondrogenic differentiation culture method is established to provide mechanical evidence for using a bioreactor in vitro in cartilage tissue engineering to perform large-scale amplification culture on cartilage seed cells and provide a basis for further studying a mechanical signal transduction mechanism in human umbilical cord mesenchymal stem cell chondrogenic differentiation under a stress loading condition.
Year: 2019
Country: CN
Doc No: 109161523