23 Jul Human single chain antibody and application thereof
Abstract
The invention belongs to the field of antibody medicine, discloses human single chain antibody namely PHD2-resistant human single chain antibody INP and HD2-resistant human intracellular single chainantibody ER-INP and discloses polynucleotide for encoding the human single chain antibody, a carrier containing the polynucleotide and application. The human single chain antibody is screened by utilizing a phage antibody library technology and prepared by utilizing a genetic engineering method. The human single chain antibody INP and ER-INP disclosed by the invention is a low-toxicity, high-efficiency and specific active molecule for inhibiting PHD2 hydroxylation activity, and toxic and side effects of heterologous antibody which is applied to a human body are avoided. Meanwhile, the human single chain antibody is micromolecular single chain antibody and has the advantages of small molecular weight, low immunogenicity and the like; in addition, endoplasmic reticulum location signals are introduced , so that the human intracellular single chain antibody ER-INP can efficiently and stably inhibit PHD2 hydroxylation activity in cells, can effectively improve HIF protein level, promotes angiogenesis damage repair of tissues like liver and the like and has a protecting effect on tissue damage.
Year: 2018
Country: CN
Doc No: 108059679