23 Jul Human il-1 cDNA sequences encoding biologically-active human il-1 proteins.
Abstract
Nucleic acids comprise a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Truncated human IL-1 cDNA sequences encode biologically-active novel human IL-1 proteins. cDNA synthesised by reverse transcription of poly(A)RNA isolated from adherent human monocytes stimulated with bacterial endotoxin, is cloned. Truncated human IL-1 cDNA sequences can be obtained by genetic engineering procedures using cloned human IL-1 cDNA as a starting material. Human IL-1 and human IL-1 proteins obtained via cloned truncated human IL-1 cDNA sequences can induce the production of IL-2 by activated T-cells; they also act on B-cells and NK-cells.Recombinant DNA cloning vehicle contg. the human interleukin-1 (IL-1) gene sequence is new. In partic., the vehicle contains the sequence coding for a new specified 287 amino acid sequence or novel fragments of it. Also claimed are (1) a pure cDNA of 1507bp (and its 1-606; 1-677; 1355-1507; 482-1501; 482-677 and 1355-1507 fragments); (2) microorganisms or mammalian tissue cells contg. these DNA sequences and (3) antibodies to human IL-1 raised using the new aminoacids as immunogens.
Year: 1985
Country: EP
Doc No: 161901