23 Jul HUMAN BICUNIN (VARIANTS), DNA ENCODING ITS AND EXPRESSION VECTOR (VARIANTS)
Abstract
FIELD: molecular biology, genetic engineering. SUBSTANCE: protein (bicunin) is identified in human placenta tissue that comprises two domains typical for inhibitors of serine proteases of Kunitz’s family and shows inhibitory activity with respect to human trypsin and plasmin. Its activity is comparable with that of aprotinine but expressed more significantly by effect on kallikrein as compared with aprotinine. Invention gives DNA sequence encoding human bicunin and amino acid sequence of protein and proposes also vectors for expression providing possibility for preparing inhibitor in heterogenous systems. By synthetic and recombinant methods different fragments and derivatives of bicunin are prepared, among them that containing part of transmembrane domain that retain, at least part, of activity of full-scale native protein. Invention can be used in medicine. EFFECT: valuable biological and medicinal properties of bicunin. 6 cl, 24 dwg, 11 tbl, 10 ex
Year: 2003
Country: RU
Doc No: 2218353