23 Jul Human amniotic membrane tissue-derived mesenchymal cells extract method and subcultring method
Abstract
The invention relates to a human amniotic membrane tissue-derived mesenchymal cells extract method and a subcultring method, belonging to the regenerative medical engineering field. The extract method of amniotic membrane tissue-derived mesenchymal cells hAMCs comprises the steps of removing amniotic epithelial cells and separating and purifying mesenchymal cells and is characterized in that in the step of separating and purifying mesenchymal cells 0.1% collagenase III, IV and V are adopted for the digestion treatment; substrate DMEM/F12 is adopted in the in vitro subcultring method of mesenchymal cells hAMCs, and the supernate of amniotic epithelial cells culture solution with growth factor is added in the substrate. The extract method of the invention has the advantages that the degree of separation is high so that the maximum of 1*10/cell of cells can be obtained in 30ml of amniotic membrane issues and the cell survival rate is more than 90%. By using the subcultring method of the invention, the proliferation of mesenchymal cells can be fast and excellent and the proliferation tend can exist after culturing for 35d.
Year: 2009
Country: CN
Doc No: 101591643