23 Jul Honeysuckle tissue culturing and honeysuckle tissue culture medium preparation method
Abstract
The invention discloses a honeysuckle tissue culturing and honeysuckle tissue culture medium preparation method, relates to a plant tissue culturing and plant tissue culture medium preparation method and belongs to the field of biological cell engineering technology. The honeysuckle tissue culturing and honeysuckle tissue culture medium preparation method is characterized by including the steps of taking embryos of honeysuckle to disinfect, culturing the embryos of the honeysuckle on a culture medium under the temperature of 25+/-1 DEG C and illumination of 2000Lux for 60 days until multiple shoots generate, shifting the multiple shoots into a test tube similar to the culture medium, culturing the multiple shoots under the temperature of 25+/-1 DEG C and illumination of 2000Lux for 10-15 days to induct the multiple shoots to take roots. The honeysuckle tissue culturing and honeysuckle tissue culture medium preparation method has the advantages of being free of influence of seasons and climate, and quite suitable for volume and large-scale production of honeysuckle seedlings all year around. Through adoption of the method, honeysuckle test-tube seedlings are generated from explants via a callus stage. Therefore, the method is labor-saving, easy to operate and capable of effectively preventing insufficient variation caused by calluses.
Year: 2013
Country: CN
Doc No: 103125379