23 Jul High-throughput single cell capture and arrangement chip and method
Abstract
The invention relates to a high-throughput single cell capture and arrangement chip and method and belongs to the field of materials and tissue engineering and particularly relates to high-throughputcapture and arrangement of single cells. The method comprises the following steps: making a substrate surface micro-pattern under the action of protein penetration, performing the high-throughput capture and arrangement of the single cells under the action of membrane pore limitation, and arranging and paving the cells on the complex micro-pattern. The high-throughput single cell capture and arrangement chip and method have the benefits that the capture rate of 85 percent or above of single cells can be realized, and the orderly arrangement and the orderly pavement of the cells on the specified protein pattern are realized; the influence caused by cellular heterogeneity during the large cell population study is avoided, and the method is simple and high in single cell capture rate, has a suitable follow-up culture environment, can be determined to be applied to the study on cell junction, intercellular interaction, cell signal transduction and the like at a single cell level and has avery important effect in the cell engineering and tissue engineering study.
Year: 2018
Country: CN
Doc No: 108165475