23 Jul High-efficiency tissue culture and regeneration method for Medicago sativa
Abstract
The invention discloses a high-efficiency tissue culture and regeneration method for Medicago sativa, which sequentially comprises the following steps of: (1) culturing sterile seedlings; (2) inoculating leaves of the Medicago sativa sterile seedlings to a callus induction culture medium for culturing; (3) transferring calli to a subculture medium 1 for culturing; (4) transferring the calli of which green shoots are to be grown to a callus subculture medium 2 for subculturing; (5) selecting the calli which grow well, and then transferring the calli to a differential medium for performing differentiated culture; and (6) after differentiation and emergence, transferring the seedlings to a rooting medium for performing rooting culture. The method has the advantages that: the high-efficiency regeneration of plants in the tissue culture process of the Medicago sativa is realized by adding an appropriate dose of TDZ and subculturing continuously; and by the method, the differentiation rate of the Medicago sativa calli is averagely 12.3 percent higher than that of the prior art. The method has the advantages of simple operation, and high regeneration success rate of the plants; and the method lays the foundation for genetic improvement by further using genetic engineering.
Year: 2011
Country: CN
Doc No: 101946711