23 Jul Heterogeneous dermis reticular layer stent without basement membrane and cell as well as preparation method thereof
Abstract
The invention discloses an xenogenic dermal reticular layer scaffold with basilar membrane removal and no cells, which is taken from an dermal reticular layer of a mammal, and has a natural three-dimension structure with larger pores of the dermal reticular layer; the dermal scaffold removes a basilar membrane and has no cells; and the thickness of the dermal scaffold is between 0.2 and 0.8mm. The scaffold is prepared by performing sterile dermis getting, then removing subcutaneous tissue, epidermis, the basilar membrane and a dermal papillary layer, then orderly using distilled water to soak and using trypsinase and an EDTA.Na2 digestive juice to digest, using a phosphate buffer solution to wash after the persistent oscillation, then placing into a Triton X-100 solution for soaking and performing the persistent oscillation, and then using the phosphate buffer solution to wash. The scaffold completely removes the source of a main immunogenic substance in dermis, and reduces the immunogenicity; the scaffold has strong nutrition permeability, quick vascularization speed, high survival rate and good effect when used for the repair of skin wounds, the construction of tissue engineering skin, and tissue filling; and the scaffold has the advantages of rich dermis sources, simple preparation technology, short production period, and low cost.
Year: 2009
Country: CN
Doc No: 101496915