23 Jul Halimodendron halodendron ERF transcription factor cDNA sequence, expression vector and use thereof
Abstract
The invention discloses a cDNA sequence of ERF transcription factor from Halimodendron halodendron, as well as an expression vector and application thereof, which belongs to the field of plant genetic engineering. A novel ERF2 ERF2 transcription factor gene HhERF2 (SEQ ID NO:1) is obtained by separating and cloning psammophyte Halimodendron halodendron through a RACE-PCR technique. The result of tissue-specific expression shows that the gene is expressed in roots, stalks and leaves of the Halimodendron halodendron. The result of stress response detection shows that the expression of the gene is induced by droughty, high-salt and low-temperature environmental stress and does not depend on the regulation of ABA. The cDNA sequence is transformed into Arabidopsis for expression; the stress resistance of transgenic Arabidopsis to drought, high salt, low temperature, diseases and the like is analyzed; and the relevant physiological and biochemical indexes of the transgenic Arabidopsis are determined. Experimental result shows that the cDNA sequence can effectively improve the drought resistance, osmotic-stress resistance and disease resistance of plants.
Year: 2009
Country: CN
Doc No: 101503693