23 Jul Growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material
Abstract
The invention discloses a growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material. A method for preparing the growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material includes the following steps: (1) preparing a DBM: adopting a fresh pig shoulder blade, removing the periosteum, the cartilage and the soft tissues, taking the cancellous bone of the shoulder blade, preparing the cancellous bone to be in a cake shape, carrying out repeated stirring and washing with flowing water to remove the marrow, the bloodiness and the surface grease, and after washing is carried out through the tap water, placing the cancellous bone into an environment at -80 DEG C for storage for three days to carry out proper processing; (2) preparing chitosan sustained release microspheres; (3) preparing wrapping growth factor microspheres, and calculating the encapsulation efficiency and the drug loading capacity; (4) preparing a growth-factor-chitosan-sustained-release-microsphere loaded DBM composite, and measuring amido bond connection after cross bonding is carried out a DBM support and the microspheres through EDC with the infrared ray method. The growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material has the good cellular and biology compatibility and the good degradability, and is a good cartilage tissue engineering support material.
Year: 2014
Country: CN
Doc No: 104056304