23 Jul Grass carp interleukin 21 recombinant protein and preparation method thereof
Abstract
The invention relates to a grass carp interleukin 21 recombinant protein and a preparation method thereof. According to the invention, a homologous cloning technology is used to obtain a coding sequence of grass carp interleukin 21 protein from grass carp gill tissue through cloning; protein mature peptide recombinant expression plasmid is constructed, is conversed into bacteria, and is cloned toobtain engineering bacterial strain; the obtained engineering bacterial strain is subjected to inducible expression through a derivant, and then is subjected to separation and purification, denaturation and renaturation to obtain the active recombinant grass carp interleukin 21 protein. The recombinant protein with high purity is obtained, at the same time, an affinity chromatography step is reduced, the target protein flow and cost can be greatly simplified, the purity can achieve as high as 95%, the grass carp interleukin 21 recombinant protein has high active protein yield, the operation issimple and easy to operate, and the preparation cost is low.
Year: 2018
Country: CN
Doc No: 108822202