23 Jul Gene LcGST for enhancing transgenic organism salt tolerance, preparation thereof and protein coded by using same
Abstract
The invention belongs to preparation of new gene, and particularly relates to a gene LcGST for enhancing transgenic organism salt tolerance and a protein coded by using the same. An outer forward primer is 5′-GTTCTTCCTCAAACTTCAACC-3′; an outer reverse primer is 5′-TTTCAAACCCTGTACAATCG-3′; an inner forward primer is 5′-ATAGGTACCGAGCTCACCATGGCTGACGAGGTG-3′; and an inner reverse primer is 5′-CTGGGATCCCTCGAGCTACTCGATGCCTAACTTG-3′. Leaf tissue cDNA, which is obtained by culturing crowtoe for 21 days, is used as a template, glutathione S-transferase gene LcGST is obtained in the crowtoe by using a nest type PCR amplification method, and the gene LcGST is used for coding the protein capable of enhancing the transgenic organism salt tolerance. The invention provides a new candidate gene for salt tolerance gene engineering. The glutathione S-transferase gene LcGST can be utilized to lay the foundation for the further transfer to important crops to enhance transgenic plant salt tolerance, and has high economic benefit and application value.
Year: 2011
Country: CN
Doc No: 101942468