23 Jul Gene for detecting microRNA-34 of living cells in situ and preparation method and application thereof
Abstract
The invention provides a gene for detecting microRNA-34 of living cells in situ and relates to the technical field of genetic engineering. The gene realizes rapid detection of microRNAs in situ on thebasis of a DNA stereoscopic tweezer structure, detection in the living cells can be realized rapidly, and a series of operation such as microRNA extraction, reverse transcription and PCR is avoided.Meanwhile, in order to deal with the current situation that the specificity of microRNAs for disease diagnosis is poor, in-situ detection can well realize the detection of cells with abnormal microRNAexpression, so that the sources of cells and tissue with diseases are determined, and the clinical significance of microRNA detection can be greatly enhanced. In the embodiment, the to-be-detected microRNA-34 which is subjected to gradient dilution is detected, the detection limit reaches 1.499 nm, and the sensitivity is high. Meanwhile, compared with other microRNAs with similar sequences, the microRNA-34 shows high specificity.
Year: 2019
Country: CN
Doc No: 110484605