23 Jul Gene cloning, vector construction and application of baical skullcap root anthocyanin transcriptional regulation factors SbMYB75 and SbDEL
Abstract
The invention discloses a baical skullcap root anthocyanin transcriptional regulation factor, which comprises at least one of a SbMYB75 gene and a SbDEL gene, wherein a SbMYB75 gene sequence is shownas SEQ ID No.1, and a SbDEL gene sequence is shown as SEQ ID No.3. The invention also provides a primer composition for amplifying the above genes, a protein encoded by the genes, a recombinant vector, a recombinant microorganism, a host cell, a transgenic cell line or a transgenic plant tissue constructed by the above genes and an application thereof. Baical skullcap root anthocyanin transcription factors SbMYB75 and SbDEL and the recombinant vector constructed by the baical skullcap root anthocyanin transcription factors SbMYB75 and SbDEL are utilized to improve anthocyanin yield of the transgenic cell line. According to the gene cloning, vector construction and the application of the baical skullcap root anthocyanin transcriptional regulation factors SbMYB75 and SbDEL, the anthocyanin transcription factors SbMYB75 and SbDEL and encoded proteins thereof are cloned from baical skullcap root for the first time, the transgenic cell lines with high anthocyanin content are obtained through genetic engineering and cell engineering, and a solid foundation is laid for industrial improvement of the anthocyanin yield.
Year: 2019
Country: CN
Doc No: 109943575