23 Jul Gas-liquid level growth medium for melanin and keratinocytes for constructing reconstituted skin model
Abstract
The invention provides a gas-liquid level growth medium for melanin and keratinocytes for constructing a reconstituted skin model and relates to the technical field of tissue engineering. Gathered and abnormally germinated melanin model tissue sections prove that the main reason lies in non-uniform melanin germination and excessive germination of the keratinocytes. The growth medium has the characteristics of promoting uniform melanocytes germination and inhibiting excessive germination of the keratinocytes, and attachment and proliferation of melanocytes are promoted by adding cholera toxin and TPA (12-O-tetradecanoylphorbol-13-acetate). In terms of 500 ml, the gas-liquid level growth medium for the melanin and the keratinocytes for constructing the reconstituted skin model comprises 500 ml of cutin serum-free medium K-SFM, 2-30 mu g of insulin, 10-800 mu g of hydrocortisone, 10-200mg of bovine pituitary extract, 20-2000 mu g of an upper epidermal growth factor, 1-1500 mu g of a basic fibroblast growth factor, 5-80 mu g of a keratinocyte growth factor, 20-200 ng of TPA, 10-200 mg of CaCl2 and 20-200 nM of cholera toxin.
Year: 2017
Country: CN
Doc No: 107326004