23 Jul Fusion protein of fibronectin and cadherin-11, preparation and use thereof
Abstract
The invention relates to a fusion protein of fibronectin and cadherin-11 as well as a preparation method and application thereof, aims at providing a fusion protein capable of promoting the adhesion between bone and cartilage seed cells and a scaffold material, and belongs to the tissue engineering field. The method comprises the following steps: employing an FNIII7-10 fragment containing PHSRN and RGD motif and cadherin-11 ectodomain EC1-2 to prepare the fusion protein, performing surface modification on the a biological scaffold material, taking full account of adhesion ‘isophil’ and ‘heterophil’ of the seed cells, and compromising the adhesion of the seed cells and differentiation of osteogenesis and the cartilage to establish a surface biomimetic microenvironment of bone and cartilage tissue engineering biomaterials. The fusion protein is applicable to the construction of tissue engineering bones and cartilage products and the manufacture of cell-loaded plastic materials.
Year: 2009
Country: CN
Doc No: 101463090