23 Jul Fibrous membrane for non-virus gene treatment and preparation method thereof
Abstract
The invention relates to a fibrous membrane which is applied to tissue engineering mediated non-virus gene treatment and preparation method thereof. The fibrous membrane comprises an organic high polymer, growth factors and a small molecular pore-forming agent, and is prepared by performing electrostatic spinning on an outer layer organic high polymer solution and an inner layer growth factor solution by needle heads which are arranged in a concentric mode and have different diameters, wherein the small molecular pore-forming agent is added into the outer layer organic high polymer solution. The fibrous membrane has the advantages that: a composite fiber material with a nuclear-shell structure is prepared by a coaxial electrostatic spinning method, so that the encapsulating rate of electric spinning fibers is improved, and the bioactivity of the growth factors can be kept for a long time; the sustained release of the growth factors is facilitated by the small molecular pore-forming agent, and blood coagulation factors can be released stably for a long time; and various growth factors can promote tissues and designed skeletal muscles to be well integrated, so that an effect of treating A type hemophilia for a long time is achieved, and the fibrous membrane can be applied to the tissue engineering mediated non-virus gene treatment.
Year: 2012
Country: CN
Doc No: 102499996