23 Jul Fast culturing method for lily virus-free seed ball
Abstract
The invention discloses a comprehensive virus-free technique for manufacturing lily seed balls, namely, thermally treating and planting lily seed balls for 7-15 days at 35-38 degrees centigrade; the stem tip growing points of the thermally treated lily seed balls are taken as explants; vaccinating the explants in a culture medium, of which the Ribavirin content is 5-10mg/L, to be in virus-free cultivation; and detecting the viruses, such as the lily symptomless virus (LSV), the cucumber mosaic virus (CMV) and lily mottle virus (LMoV) of virus-free tissue culture seedling by the enzyme-linked immunosorbent assay test(ELISA) and the RT-PCR (Reverse Transcription-polymerase Chain Reaction) detection; the fast culturing method for the lily virus-free seed ball ensures that the cultured lily seed balls remove the virus infection through the comprehensive virus-free technique; the tissue culture medium component and culture program of the virus-free lily seed balls are optimized; virus-free cultivation seed balls are obtained through fast cultivation; and the fast culturing method belongs to the fields of plant culture rapid propagation technique and plant cell engineering technique.
Year: 2012
Country: CN
Doc No: 102440185