23 Jul F protein detection reagent and detection method therefor
Abstract
The invention relates to an F protein detection reagent and a detection method therefor. The F protein detection reagent comprises rabbit anti-human polyclonal antibodies of an F protein, enzyme-labeled antibodies, color reagents and a stop solution. The enzyme-labeled antibodies are horse radish peroxidase-labeled antibodies. The color reagents comprise a solution A containing 0.01% of H2O2, and a solution B which is 0.1mol/L of a citric acid buffer having a pH value of 7.4 and containing 0.4mg/ml of TMB (3,3,5,5- tetramethylbenzidine). The stop solution is 2M of a sulfuric acid aqueous solution. After liver tissue is damaged, a large amount of F proteins are released from liver cells into blood circulation thereby inducing an organism to produce specific cellular and humoral immune responses. Based on the characteristic, an HIS-F protein prepared by a gene engineering technology and highly purified by Ni<2+> column chromatography is adopted so that the diagnosis precision and the diagnosis efficiency are improved and a detection cost is saved. Therefore, the F protein detection reagent has an important theoretical significance and a potential application value in clinical application.
Year: 2012
Country: CN
Doc No: 102721814