23 Jul Extraction and purification method of high-quality enlarged fish collagen
Abstract
The invention discloses an extraction and purification method of high-quality enlarged fish collagen and belongs to the technical field of extraction and purification of fish collagen. The method comprises the following steps: after cutting freeze-dried fish skin, adding a mixed solution of a sodium hydroxide solution and a hydrogen peroxide solution; after stirring, centrifuging; adding an isopropyl alcohol solution into sediment; after stirring, centrifuging; adding a guanidine hydrochloride solution into the sediment and stirring and centrifuging; adding an acetic acid solution into the sediment; then adding pepsin; after stirring, centrifuging; dropwsie adding a sodium chloride-acetic acid mother solution into supernatant while stirring; after stirring, centrifuging; adding the aceticacid solution into the sediment; after dissolving the sediment, dialyzing; after dialyzing, dissolving sediment in a dialysis bag into the acetic acid solution; adding a mixture into positive-pressurefiltering equipment; filtering supernatant with a micro-pore filtration membrane to obtain a protein solution; dialyzing with the acetic acid solution; then dialyzing with water to obtain collagen gel; after carrying out freeze drying, thus obtaining the collagen. An obtained protein product has a complete structure and the bioactivity keeps intact; the protein product can be directly used for medical and tissue engineering materials.
Year: 2018
Country: CN
Doc No: 108642115