23 Jul Extracellular matrix biomaterial for resisting cell premature senility and preparation method and application thereof
Abstract
The present invention discloses an extracellular matrix biomaterial for resisting cell premature senility and a preparation method and application thereof. The matrix is secreted from culture of umbilical cord mesenchymal stem cells and is resistant to cell premature senility. The umbilical cord mesenchymal stem cells are cultured in a complete medium; when the cell density is close to 90%, a complete culture solution containing ascorbic acid is added, and culture is carried out for 7-10 days; then a cell removal solution is added to construct an extracellular matrix biomaterial. The umbilical cord mesenchymal stem cells are obtained from human source, swine source, murine source or rabbit source. The extracellular matrix biomaterial for resisting cell premature senility can be applied to cell culture, including cell transplantation therapy, regenerative medicine and tissue engineering, etc. Studies show that the intracellular MSCs cultured in ECM environment has ROS content significantly decreased, which confirms that ECM from UC-MSCs can alleviate the UC-MSCs premature senility induced by H2O2 and provide a new policy to address the challenges brought by cells aging.
Year: 2016
Country: CN
Doc No: 105854084