23 Jul External Cultivating method for bird primordial germ cell
Abstract
The external cultivating method of primordial bird germ cell relates to transgene, animal cloning, rare animal preservation, tissue and cell engineering and other science fields. After it is inoculated to the CEF raising layer, the primordial bird germ cell is cultivated in liquid high-sugar DMEM cultivation medium with added calf serum in 10 úÑ, chicken serum in 2 úÑ, L-glutamine in 2mmol/L, sodium pyrovate in 1 mmol/L, beta ¿Cmercaptoethanol in 5.5í 10-5 mol/L, optional amino acid 10 microliter/ml, hSCF in 15-20 ng/ml, mLIF in 10 U/ml, bFGF in 10-15 ng/ml, hIL-11 in 0.04 ng/ml, IGF in 10 ng/ml, and gentamycin sulfate in 100 U/ml. The present invention has simple operation and high repeatability, and lays the foundation of the relevant research.
Year: 2006
Country: CN
Doc No: 1821389