23 Jul Experimental method for promoting tissue engineering pre-vascularization by umbilical cord mesenchymal stem cells
Abstract
The invention discloses an experimental method for promoting tissue engineering pre-vascularization by umbilical cord mesenchymal stem cells; according to the method, vascular endothelial cells and umbilical cord mesenchymal stem cells with different proportions are co-cultured in a two-dimensional manner, and a rule of promoting vascular endothelial cell proliferation and hematopoietic differentiation by the umbilical cord mesenchymal stem cells is studied. In addition, a micron-sized network microvascular scaffold is prepared by high temperature molten monosaccharide and is dissolved in hydrogel to construct a continuous microchannel culture system of a three-dimensional hydrogel female die, the two cells are subjected to perfusion and suspension culture, an in-vitro constructed microvascular network structure and an engineered bone tissue in-vivo transplantation effect based on the structure are evaluated, and a mechanism of the two cells participating and promoting microvascular network construction is illuminated. A new solution and an experimental basis are provided for in-vitro pre-vascularization of engineered tissues and organs.
Year: 2018
Country: CN
Doc No: 107748260