23 Jul Establishment method of kiwi fruit genetic transformation receptor system based on heat stress
Abstract
The invention relates to the technical field of plant tissue culture and gene engineering, and in particular relates to an establishment method of a kiwi fruit genetic transformation receptor system based on heat stress. The establishment method comprises the following steps: by utilizing Chinese gooseberry Funiu95-2 tissue culture seedling lamina subjected to heat stress treatment as an explant, processing the explant to obtain a callus tissue, and performing adventitious bud induction culture and rooting culture. The method is simple and feasible in operation, and is capable of greatly shortening the regeneration time of an original transformation receptor system; meanwhile, the differentiation ration of adventitious buds of the callus tissue is further improved, and the root development capability of adventitious buds is also greatly improved.
Year: 2016
Country: CN
Doc No: 105557523