23 Jul ENGINEERING FUNCTIONAL TISSUE FROM CULTURED CELLS
Abstract
Described herein is a method for islet cell engineering or regeneration in a subject, wherein a combination of pancreatic islet cells are cultured on a scaffold to form a cell aggregate, and subsequently suspended in a hydrogel and cultured under growth conditions sufficient to form a pancreatic islet. After sufficient growth and/or differentiation of the cells, an engineered islet is isolated from the scaffold and the hydrogel. Engineered islets produced in this manner are functional and can be used as in organ replacement, or augmentation of function. For example, described herein are methods and compositions suitable for producing a functional pancreatic islet for implantation into a subject having Type 1 diabetes.
Year: 2010
Country: WO
Doc No: 2010068728