23 Jul Elaboration of artificial fabrics through tissue engineering using fibrine and agarose biomaterials. (Machine-translation by Google Translate, not legally binding)
Abstract
Elaboration of artificial tissues by tissue engineering using fibrin and agarose biomaterials. The present invention provides an in vitro method of preparing an artificial tissue, the artificial tissue obtainable by said method, the pharmaceutical composition comprising it and the use of this tissue or of this composition to increase, restore or partially or totally replace the activity functional of a damaged tissue or organ, preferably skin, bladder, urethra, cornea, oral mucosa, conjunctiva, abdominal wall, tympanic membrane, pharynx, larynx, intestine, peritoneum, ligament, tendon, bone, meninx or vagina. The method of the invention comprises the steps of adding fibrinogen to isolated cells, preferably to fibroblasts or keratocytes, adding an antifibrinolytic agent to the mixture, adding in addition a coagulation factor, a calcium source and thrombin, adding a polysaccharide, preferably agarose., culturing cells, preferably epithelial cells or stem cells of the umbilical cord, in or on the resulting product and inducing the nanostructuring of said product. (Machine-translation by Google Translate, not legally binding)
Year: 2011
Country: ES
Doc No: 2353990