23 Jul Efficient regeneration method with zenia insignis leaves being explants
Abstract
The invention discloses an efficient regeneration method with zenia insignis leaves being explants and belongs to the technical field of plant tissue culturing. With the leaves being materials, a zenia insignis regeneration system is researched for improvement in the regeneration capacity of zenia insignis, and an efficient regeneration system is established and used for providing effective reference value for genetic resource conservation and genetic engineering research later. The efficient tissue culturing and plant regeneration method can be free of influences of external conditions, and robust, neat and uniform zenia insignis tissue culture seedlings are produced on a large scale in an industrialized mode. The zenia insignis leaves serve as the drawn materials, and compared with the reported mode that hypocotyls and annual branches serve as explants to construct a regeneration system, the efficient regeneration method has the advantages of being convenient to operate, wide in range of drawn materials, high in repeatability and efficiency and the like. The method not only can meet the requirement for seedlings of the market, but also can modify and innovate zenia insignis genetic resources through transgene research to lay a foundation for sustainable utilization of the zenia insignis genetic resources.
Year: 2017
Country: CN
Doc No: 106305416