23 Jul E.urophylla*E.saligna rooting culture medium and application thereof
Abstract
The invention belongs to the fields of forestry biotechnological seed breeding or plant cell engineering and discloses an E.urophylla*E.saligna rooting culture medium and an application thereof. On the basis of an improved MS culture medium, IBA and 3-AB are supplied to the rooting culture medium. By means of addition of the novel rooting inductive substance 3-AB and the synergistic effect of the rooting-type growth regulator IBA, rooting ratio of the E.urophylla*E.saligna is more than 87% and transplanting survival rate is more than 91%. When rooted seedlings are transplanted on bags, the seedlings grow tidily and robustly, have strong resistance, and completely reach demands of large-scale and commercial seedling production. The rooting culture medium has good repeatability and stable effects, and has no intensive fluctuation on the rooting ratio and transplanting survival rate even after multiple times of usage, thereby completely solving a problem that the E.urophylla*E.saligna is difficult to root during tissue culture induction.
Year: 2017
Country: CN
Doc No: 106359085