23 Jul Double-layer collagen-chitosan sponges bracket and method of preparing the same
Abstract
The invention discloses a dual-layer collagen-chitosan sponge scaffold and a preparation method thereof, and relates to a tissue engineering scaffold. The invention provides the dual-layer collagen-chitosan sponge scaffold for the tissue engineering skin and the preparation method thereof. The scaffold is a dual-layer mesh structure, the upper and the lower layers are collagen and chitosan composite films, and the hole diameters of the layered and hole-shaped structure are mutually penetrated. The collagen hydration solution is dissolved in acetic acid for preparing the collagen dispersion solution with the concentration of 0.2 percent to 0.5 percent and 0.5 percent to 0.8 percent; the chitosan is dissolved in the acetic acid for preparing the chitosan dispersion solution with the concentration of 0.2 percent to 0.5 percent and 0.5 percent to 0.8 percent; the two dispersion solutions are mixed to obtain two collagen-chitosan solutions; the collagen-chitosan solution with the concentration of 0.5 percent to 0.8 percent is frozen overnight, the sponge-like white scaffold is obtained by drying, and the single-layer scaffold is obtained by cross-linking after the film pressing, cleaning, freezing overnight and drying; the collagen-chitosan solution is poured in for freezing overnight and drying to obtain the dual-layer scaffold, and the cross-linking, the cleaning, the freezing overnight, the freezing and the drying are carried out.
Year: 2008
Country: CN
Doc No: 101254313