23 Jul Detection method of promoted cell proliferation rate of freeze-dried powder
Abstract
The invention relates to the technical field of tissue engineering, and in particular relates to a detection method of a promoted cell proliferation rate of freeze-dried powder. The method can be used for accurately detecting the promoted cell proliferation rate of freeze-dried powder. The embodiments of the invention provide a detection method of the promoted cell proliferation rate of freeze-dried powder. The detection method comprises the following steps: 1) dissolving to-be-detected freeze-dried powder in a complete culture medium to obtain a sample culture medium which has a first osmotic pressure; 2) by taking the complete culture medium without the to-be-detected freeze-dried powder as a control medium, adjusting the osmotic pressure of the control medium to be equal to the first osmotic pressure; 3) carrying out culture on the same number of cells under the same condition by using the obtained sample culture medium and the control medium respectively, and obtaining sample cells and control cells after a first preset time; and 4) carrying out MTT (methylthiazolyldiphenyl-tetrazolium bromide) dyeing and light absorption value detection on the obtained sample cells and control cells, and comparing the light absorption values of the sample cells and the control cells to obtain the promoted cell proliferation rate of a sample in respect to a control.
Year: 2016
Country: CN
Doc No: 105543322