23 Jul Design of cancellous bone bionic scaffold prepared by 3d printing technology and application thereof
Abstract
The invention relates to a novel bionic scaffold model constructed through a 3D-Voronoi algorithm and a random distribution micropore algorithm, and a high-precision tissue engineering scaffold is manufactured by using a biological 3D printing technology. The bovine femur is calcined by using an ammonium dihydrogen phosphate secondary calcination method, inorganic components such as hydroxyapatiteand the like are removed on the basis of realizing allogeneic bone degreasing, decalcification and antigen removal, so that the remainder is high-purity beta-tricalcium phosphate, and variable difference caused by a matrix material in an experiment is eliminated while the form of a natural cancellous bone is retained. Through various experiments such as characterization detection, structural analysis, in-vitro experiments and in-vivo implantation, the influence of different scaffold structures on cell proliferation and adhesion and in-vivo bone repair effects is comprehensively evaluated, a reliable scaffold model and an effective detection means are provided for bionic structure design of the bone tissue engineering scaffold, and the bone tissue engineering scaffold can be used for clinically treating various bone defects.
Year: 2019
Country: CN
Doc No: 110559483