23 Jul Culture of tissue engineering skin and detection method of proliferation activity
Abstract
The invention discloses a detection method of tissue engineering skin culture and proliferation activity, which comprises the following process: first three-dimensional host materials containing basilar membrane are prepared as the substitute of dermis, namely dermis tissue of human with epidermis being removed, and keratinocyte is cultivated; the keratinocyte is inoculated to the surface of the dermis tissue of human with epidermis being removed and coated with mixed tissue glue, sampling is carried out for the detection of the skin proliferation activity after the culture of air-liquid surface culture mode, the culture is stopped after the culture is continued for a week, and then the skin is picked out for skin proliferation activity detection. A fresh culture specimen picked is put into BrdU culture liquid and a culture box of 37 DEG C for breeding, immnohistochemical staining is carried out after 24 hours, proliferation cells are observed under a microscope, the quantity of proliferation cells is calculated by using a computer imaging system, and tissue engineering skin structure is observed by the method of histology simultaneously. The detection method of tissue engineering skin culture and proliferation activity can detect the impact of different culture conditions and relevant factors on the tissue engineering skin cell proliferation by quantization.
Year: 2009
Country: CN
Doc No: 101392237