23 Jul Culture method of stanuntonia chinensis germchits
Abstract
The invention belongs to the technical field of plant cell engineering. A culture method of stanuntonia chinensis germchits sequentially comprises the following steps: pre-treating, obtaining sterile explants, carrying out callus induction culture, carrying out multiplication and rooting culture on cluster buds and embryoids and transplanting. With the adoption of the technical scheme for producing the stanuntonia chinensis germchits, the culture method has the beneficial effects that (1) the multiplication rate is high, the biological yield is great and the culture period is short; (2) the quality of tissue culture seedlings is good, the germchits are strong and the culture survival rate is high; the stanuntonia chinensis germchits are cultured by using the method comprising the steps of pre-treating, obtaining the sterile explants, carrying out the callus induction culture, carrying out multiplication and rooting culture on the cluster buds and the embryoids and transplanting; compared with other culture manners, the culture method has high multiplication efficiency, short culture time and convenience for transplanting; the germchits are strong, the culture survival rate is high and the cost is low; and therefore, the culture method is suitable for large-scale culture and production on the stanuntonia chinensis germchits.
Year: 2015
Country: CN
Doc No: 104782500