23 Jul Culture method of holboellia fargesii reaub germchits
Abstract
The invention belongs to the technical field of plant cell engineering. A culture method of holboellia fargesii reaub germchits sequentially comprises the following steps: pre-treating, obtaining sterile explants, inducing calluses, carrying out induction and multiplication on cluster buds, carrying out rooting culture and transplanting. With the adoption of the technical scheme for producing the holboellia fargesii reaub germchits, the culture method has the beneficial effects that (1) the multiplication rate is high, the biological yield is great and the culture period is short; (2) the quality of tissue culture seedlings is good, the germchits are strong and the culture survival rate is high; the holboellia fargesii reaub germchits are cultured by using the method comprising the steps of pre-treating, obtaining the sterile explants, inducing the calluses, carrying out induction and multiplication on the cluster buds, carrying out rooting culture and transplanting; compared with other culture manners, the culture method has high multiplication efficiency, short culture time and convenience for transplanting; the germchits are strong, the culture survival rate is high and the cost is low; and therefore, the culture method is suitable for large-scale culture and production on the holboellia fargesii reaub germchits.
Year: 2015
Country: CN
Doc No: 104782501