23 Jul Culture method for composition of periosteal biological scaffold and allogenic seed cells
Abstract
The invention discloses a culture method for the composition of a periosteal biological scaffold and allogenic seed cells. The culture method comprises the following steps: the non-immunogenic periosteal biological scaffold and the seed periosteal cells are prepared; the non-immunogenic periosteal biological scaffold is sealed after low-temperature drying; after gamma-ray disinfection of the non-immunogenic periosteal biological scaffold, the seed periosteal cells, with the concentration of 1*106 per milliliter, are dripped on the surface of the disinfected scaffold; a culture medium is added after the periosteal cells gradually permeate into the non-immunogenic periosteal biological scaffold together with a suspension, the medium change is carried out every 2 to 3 days, and the in-vitro composite culture lasts for one week. The periosteal biological scaffold obtained through the method has the characteristics of complete removal of immunogenic cells, as well as good preservation of the structure and major components of extracellular matrix; effective composition of the allogenic periosteal cells and the biological scaffold can be achieved, so that a biological composite scaffold material can be provided for tissue engineering researches on bone defects and bone nonunion.
Year: 2015
Country: CN
Doc No: 104511052