23 Jul Culture method for akebiaquinata decne seedlings
Abstract
The invention belongs to the technical field of plant cell engineering. Culture steps of akebiaquinata decne seedlings sequentially comprise pretreatment, aseptic explant obtaining, bud tissue culture, callus induced proliferation, embryoid induced proliferation, rooting culture and transplanting. The technical scheme for producing the akebiaquinata decne seedlings, provided by the invention, has the beneficial effects of high proliferation ratio, high biological yield, short culture period, good quality of tissue culture seedlings, healthy seedlings and high cultivation survival rate. Compared with other culture modes, a culture method for culturing the akebiaquinata decne seedlings by using pretreatment, aseptic explant obtaining, bud tissue culture, callus induced proliferation, embryoid induced proliferation, rooting culture and transplanting, disclosed by the invention, has the advantages of high propagation rate, short culture period, convenience in transfer, healthy seedlings, high cultivation survival rate and low cost, and is suitable for scale culture and production of the akebiaquinata decne seedlings.
Year: 2015
Country: CN
Doc No: 104798688