23 Jul Culture method and use of wheat genetic transformation acceptor
Abstract
The invention belongs to the technical field of plant tissue culture and gene engineering and relates to a culture method and a use of a wheat genetic transformation acceptor as a meristem mass. Through genetic transformation of the wheat genetic transformation acceptor, a large amount of transgenic plants are obtained. The culture method comprises the following steps of carrying out gene gun bombardment on a meristem mass, carrying out basta screening, and carrying out GUS dyeing, PCR detection and herbicide resistance detection on a plant having resistance so that the transgenic plant is determined. The culture method builds a high-efficiency wheat in-vitro regeneration and genetic transformation system, and solves the problem that the existing wheat has high regeneration difficulty, low genetic transformation efficiency and poor stability. Compared with the existing common wheat young embryo, the wheat genetic transformation acceptor has the characteristics of material collection convenience, high-frequency regeneration and fast production of a large amount of transgenic plants. The meristem mass provides an effective asexual propagation system for a wheat biological study.
Year: 2013
Country: CN
Doc No: 103416301