23 Jul Culture medium for sweat gland cells and preparation method of culture medium
Abstract
The invention relates to a culture medium for sweat gland cells and a preparation method of the culture medium. The culture medium comprises a basic culture solution and an additive, wherein the basic culture solution is an SGDM culture solution; the additive comprises the following components, by the addition amount in the basic culture solution, nerve growth factors, transforming growth factors-beta, epidermal growth factors, HEPG (heparin sulfate proteoglycan) and bone morphogenetic protein 4. The culture medium can increase the probability at which ips cells are induced into sweat gland cells, stable passage can be guaranteed, the cell proliferation speed is increased, and a source of seed cells is provided for skin tissue engineering. Additionally, no serum is used in the whole process, risks caused by animal origin pathogens are effectively avoided, and the clinical application safety is improved.
Year: 2017
Country: CN
Doc No: 106834211