23 Jul Culture medium for inducing differentiation of dental pulp stem cells into osteoblasts, and preparation method and application of culture midium
Abstract
The invention provides a culture medium for inducing differentiation of dental pulp stem cells into osteoblasts. The culture medium comprises 4-5.5g/L of bovine serum albumin (BSA), 1.5mg/L of reducedglutathione, 0.08-0.12mmol/L of beta-mercaptoethanol, 0.08%-1.5%(V/V) of SITE, 0.8-1.2M/L of beta-glycerophosphoric acid, 15-25[mu]M/L of L-ascorbic acid, 0.5-1.2mM/L of dexamethasone and the balanceof low-sugar dulbecco’s modified eagle medium (DMEM) culture medium added with an amino acid concentrated solution. The culture medium for inducing differentiation of dental pulp stem cells to osteoblasts is a serum-free culture medium, and the culture medium can be used to optimize a method for inducing differentiation of dental pulp stem cells to osteoblasts. The induction time is short and cost is low, and the invention provides a new selection for culture of seed cells in bone tissue engineering.
Year: 2020
Country: CN
Doc No: 110819586