23 Jul Cross-linked hyaluronic acid cell-scaffold material and preparation method and application
Abstract
The invention relates to a cross-linked hyaluronic acid cell-scaffold material and its preparation method and application. The cross-linked hyaluronic acid cell-scaffold material is obtained by crosslinking of high-molecular hyaluronate and low-molecular hyaluronate. The proportion of hyaluronate disaccharide molecules which take part in the crosslinking is 0.5%-20%, and expansion rate of the hyaluronate disaccharide molecules in an isotonic solution is 80%-110%. The preparation method of the cell-scaffold material comprises two freeze drying steps. Hyaluronate mixed with a cross-linking agent is formed firstly; after a heating reaction, water is added for swelling to form gel; and freeze drying is then carried out to obtain the porous scaffold material. The scaffold has abundant pores, has a certain mechanical strength and pore size and has good hydroscopicity and biocompatibility. The scaffold can be used as a tissue engineering cell-scaffold in promoting cartilage injury repairing and also can be used for preparing a haemostasis anti-adhesion material.
Year: 2014
Country: CN
Doc No: 104225677