23 Jul Corn callus specific promoter and cloning method and application thereof
Abstract
The invention discloses a corn callus specific promoter and a cloning method and application thereof. A WRKY gene is obtained by utilizing bioinformatics analysis, the sequence comparison of the gene and the genome is performed, a primer is designed to perform polymerase chain reaction (PCR) and obtain the promoter region of the gene 5′, the promoter is 2880bp and has the sequence shown in the (400)1 item of the sequence table; the analysis of the promoter shows that the promoter contains a plurality of acting elements; and the promoter is subcloned to the vector with GUS gene, expression of the GUS gene is started, and the vector is transformed in rice. The staining examine of each tissue of rice shows that the specificity of the promoter can be expressed in callus, thus the promoter hasimportant application value in genetic engineering and the gene function researches.
Year: 2011
Country: CN
Doc No: 102094021