23 Jul Construction of si RNA expression vector of tissue inhibitor of metalloproteinases (TIMPs) and application in treatment of cirrhosis
Abstract
The invention discloses the construction of a si RNA expression vector of the tissue inhibitor of metalloproteinases (TIMPs) and application in treatment of cirrhosis, which belong to the technical field of genetic engineering. In the invention, according to the design principle of siRNA, a corresponding si RNA sequence is designed by selecting nucleotide sequences of TIMP-1 and TIMP-2 coding areas respectively to perform BLAST comparison, so that isogenesis with other genes is avoided; single-stranded oligonucleotides chemically synthesized and the double stranded DNA formed by annealing in vitro are connected with a eukaryotic expression vector pGeneil-1; and enzyme digesting identification and sequence assay prove that the si RNA expression vector of the TIMP-1 and TIMP-2 is constructed successfully. The vector transfects a hepatic stellate cell (HSC) through liposome and can inhibit the expression of the TIMP-1 and the TIMP-2 obviously; cirrhosis animal model assay shows that the expression vector can reduce the collagenous fiber deposition of liver tissues considerably and is expected to be used as a novel anti-hepatic fibrosis genetic therapy in clinical treatment.
Year: 2011
Country: CN
Doc No: 101955975