23 Jul Construction method of tissue engineering epidermis model
Abstract
The invention discloses a construction method of a tissue engineering epidermis model. The construction method comprises the following steps of: A. making a thermoplastic non-woven fabric into a disc-shaped support by using a hot-pressing method; B. by taking mulberry silks as raw materials, degumming, dissolving, dialyzing and concentrating so as to prepare a silk fibroin solution; C. soaking the disc-shaped non-woven fabric support by using the prepared silk fibroin solution, wherein the concentration of the silk fibroin solution is 2 to 10%, and then carrying out vacuum drying and absolute ethyl alcohol aftertreatment; D. inoculating a human epidermis cell strain Hacat, wherein the inoculum density of the Hacat cell is 10<4>-10<5>/ml, carrying out gas-liquid surface cultivation, and proliferating so as to form a lamellar structure, thus constructing the tissue engineering epidermis model. The construction method can be used for solving the shortcoming of the conventional tissue engineering epidermis model that a support is prone to shrink in a cell cultivation process; the edge of the non-woven fabric is fused and bonded by the hot-pressing of the non-woven fabric, so that the problem that the side surface of the constructed non-woven fabric leaks in detection is avoided.
Year: 2013
Country: CN
Doc No: 103446623