23 Jul Construction method of tissue engineering blood vessel
Abstract
The invention relates to a construction method of a tissue engineering blood vessel which belongs to the tissue engineering field. The construction method of the tissue engineering blood vessel includes the following steps: (1) tubular autologous living tissue bed frame material is connected to a continuous perfusion bioreactor under the vitro aseptic condition, and the outside of a vessel lumen is filled with culture medium; (2) endothelial cells obtained from the vitro culture medium is suspended at M199 or vascular endothelium-like cells generating from the induction of bone marrow mono nucleus cells are suspended at endothelial progenitor cell culture medium and are inoculated on the inner surface of the vessel lumen according to a high density planting method; (3) after the endothelial cells or the vascular endothelium-like cells are adhered, the inner cycle and the external cycle of the vessel lumen are communicated and the blood circulation is simulated to provide proper pressure stimulation and shear stress stimulation and the endothelial cells or the vascular endothelium-like cells are cultured for 5-7 days in a carbon dioxide incubator. The construction method has the advantages that perfect cell compatibility and enough mechanical strength are provided, the defects of immunological rejection and inflammation reaction, etc. caused by heterogenetic material are overcome, and the preparation time is short.
Year: 2008
Country: CN
Doc No: 101259292