23 Jul Construction method of rapid Euonymus japonicus L.f. aureo-marginatus Rehd propagation system
Abstract
The invention belongs to the technical field of plant cell engineering and discloses a method for performing tissue culture and rapid propagation by using Euonymus japonicus L.f. aureo-marginatus Rehd stem tips and segments as explants. The method comprises the steps of conducting cluster bud induction and rooting culture on the stem tips and the stem segments with lateral buds to obtain complete regenerated plants, and sequentially conducting callus induction, callus proliferation and differentiation and rooting culture on the stem segments without lateral buds so as to obtain regenerated plants. The regenerated plants can be obtained in the two ways, operation is simple, the pollution rate and browning rate are low in the culturing process, a large amount of seedlings can be obtained within a short time without seasonal and environmental limits, and the shortcoming of cutting propagation is overcome, meanwhile conditions are provided for genetic transformation and somaclone variation breeding of the seedlings, and the method has significant practical value on theoretical research and landscape application.
Year: 2016
Country: CN
Doc No: 105613288